2020-2026 Publications

[542] Biosensor-guided evolution of chalcone synthase enhances biosynthesis of natural and non-natural flavanones (2026)

E. K. R. Hanko, C. Kesornpun, J. N. Whitehead, R. Speiss, C. J. Robinson & N. S. Scrutton

Metabolic Engineering, Accepted

[541] Pockets to products: A data-driven approach for classification of monoterpene synthases (2026)

C. O'Raghallaigh, N. S. Scrutton & S. Hay

Digital Discovery

DOI: 10.1039/d6dd00312e

[540] From Discovery Proteomics to Process-Informed Monitoring in Biomanufacturing Chassis Development (2026)

M. R. Russell, P. J. Brownridge, J. Windo, N. S. Scrutton, C. E. Eyers & P. E. Barran

ACS Measurement Science Au

DOI: 10.64898/2025.12.18.695173

[539] Mechanism of Triplet Quenching in Fatty Acid Photodecarboxylase: A Generalized Approach for Enhancing Photostability (2026)

J. Ma, J. M. Kalapothakis, H. J. Spacey, L. O. Johannissen, E. Shrimpton-Phoenix, M. Shanmugam, M. Sakuma, C. W. Wood, P. E. Barran, D. J. Heyes & N. S. Scrutton

ACS Catalysis, 16, 9737 - 9745

DOI: 10.1021/acscatal.6c01233

[538] Mechanism of Photoinduced Conformational Changes in the Photoenzyme Fatty Acid Photodecarboxylase Revealed by Light- Footprinting Ion Mobility Mass Spectrometry (2026)

L. Meng, J. M. D. Kalapothakis, J. Ma, H. J. Spacey, L. O. Johannissen, A. B. Alen, D. J. Heyes, N. S. Scrutton & P. E. Barran.

J. Am. Chem. Soc. 148, 19475 - 19486

DOI: 10.1021/jacs.5c18659

[537] Integrated structural dynamics uncover a new B12 photoreceptor activation mode (2026)

R. Rios-Santacruz, H. Poddar, K. Pounot, D. J. Heyes, N. Coquelle, M. J. Mackintosh, L. O. Johannissen, S. Schianchi, L. N. Jeffreys, E. De Zitter, R. Munro, M. Appleby, D. Axford, E. V. Beale, M. J. Cliff, M. C. Davila-Miliani, S. Engilberge, G. Gotthard, K. Hadjidemetriou, S. J. O. Hardman, S. Horrell, J. S. Hub, K. Ishihara, S. Jaho, G. Karras, M. Kataoka, R. Kawakami, T. Mason, H. Okumura, S. Owada, R. L. Owen, A. Royant, A. Saaret, M. Sakuma, M. Shanmugam, H. Sugimoto, K. Tono, N. Zala, J. H. Beale, T. Tosha, J. P. Colletier, M. Levantino, S. Hay, P. M. Kozlowski, D. Leys, N. S. Scrutton, M. Weik & G. Schiro

Nature, 650, 1045-1052

DOI: 10.1038/s41586-025-10074-2

[536] Enhanced bioproduction and processing of mandelic acid enantiomers: towards a sustainable platform for high-value pharmaceutical and polymer applications (2025)

H. L. Messiha, A. Banner, M. Amer, C. J. Robinson, A. Alwattar, V. Tilakaratna, R. Le Feuvre & N. S. Scrutton

Biotechnology for Biofuels and Bioproducts, 19

DOI: 10.1186/s13068-025-02727-1

[535] Optimising environmental factors for maximal lactate productivity in Synechocystis sp. PCC 6803 through a design of experiments approach (2025)

M. Faulkner, F. Andrews & N. S. Scrutton

Biotechnology for Biofuels and Bioproducts, 18

DOI: 10.1186/s13068-025-02718-2

[534] Expanding the substrate scope of a bacterial monoterpene synthase for the production of sesquiterpenoid and diterpenoid products (2025)

N. G. H. Leferink, J. N. Whitehead, L. O. Johannissen & N. S. Scrutton

FEBS J, 293, 1341-1357

DOI: 10.1111/febs.70308

[533] De Novo Production of Xanthohumol by a Metabolically Engineered Escherichia coli (2025)

D. Gomes, J. Santos, A. Venâncio, J. L. Rodrigues, N. S. Scrutton & L. R. Rodrigues

ACS Synthetic Biology, 14, 3913 - 3926

DOI: 10.1021/acssynbio.5c00221

[532] Structural and functional insights into a novel aldehyde deformylating oxygenase with enhanced efficiency for biofuel applications (2025)

N. Treesukkasem, S. Visitsatthawong, S. Buttranon, P. Intasian, J. Jaroensuk, S. Maenpuen, J. Sucharitakul, H. Poddar, D. J. Heyes, N. S. Scrutton, P. Chaiyen & T. Wongnate

FEBS J, 293, 220 - 238

DOI: 10.1111/febs.70234

[531] The Uncatalyzed Reduction of Flavin Mononucleotide (FMN) by Nicotinamide adenine dinucleotide (NADH): A Reference Reaction for Catalysis by NAD(P)H-Dependent Flavoenzymes (2025)

J. E. Longbotham, M. Delgado, M. Yuen, J. Berreur, S. Görlich, A. Iorgu, L. O. Johannissen, D. J. Heyes, N. S. Scrutton, V. Moliner, I. Tuñón & S. Hay

ACS Catalysis 15, 14456-14468

DOI: 10.1021/acscatal.5c03307

[530] Abstraction hierarchy to define biofoundry workflows and operations for interoperable synthetic biology research and applications (2025)

H. Kim, N. J. Hillson, B. K. Cho, B. H. Sung, D. H. Lee, D. M. Kim, M. K. Oh, M. W. Chang, Y. S. Jin, S. J. Rosser, P. Vegh, R. Fragkoudis, R. Le Feuvre, N. S. Scrutton, M. Storch, W. Seong, P. S. Freemont & S. G. Lee

Nat Commun. 16, 6056

DOI: 10.1038/s41467-025-61263-6

[529] De novo production of prenylnaringenin compounds by a metabolically engineered Escherichia coli (2025)

D. Gomes, J. L. Rodrigues, N. S. Scrutton & L. R. Rodrigues

Journal of Biotechnology, 405, 215 - 228

DOI: 10.1016/j.jbiotec.2025.05.017

[528] Asymmetric Enantio-complementary Synthesis of Thioethers via Ene-Reductase-Catalyzed C–C Bond Formation (2025)

C. M. Heckmann, D. J. Heyes, M. Pabst, E. Otten, N. S. Scrutton & C. E. Paul

J. Am. Chem. Soc, 147, 18618–18625

DOI: 10.1021/jacs.5c00761

[527] Exploring the Increased Activity of the Blue Light-Dependent Photoenzyme Fatty Acid Photodecarboxylase under Violet Light (2025)

H. J. Spacey, D. Healy, J. M. D. Kalapothakis, J. Ma, M. Sakuma, P. E. Barran, D. J. Heyes & N. S. Scrutton

ACS Catal. 15, 6088-6097

DOI:10.1021/acscatal.4c07757

[526] A parallel bioreactor strategy to rapidly determine growth-coupling relationships for bioproduction: a mevalonate case study (2025)

A. Banner, J. Webb & N. S. Scrutton

Biotechnology for Biofuels and Bioproducts, 18

DOI: 10.1186/s13068-024-02599-x

[525] SignatureFinder enables sequence mining to identify cobalamin-dependent photoreceptor proteins (2024)

Y. Yu, L. N. Jeffreys, H. Poddar, A. Hill, L. Johannissen, F. Dai, M. Sakuma, D. Leys, D. J. Heyes, S. Zhang & N. S. Scrutton

FEBS J, 292, 635-652

DOI:10.1111/febs.17377

[524] Improving productivity of citramalate from CO2 by Synechocystis sp. PCC 6803 through design of experiment (2024)

M. Faulkner, F. Andrews & N. S. Scrutton

Biotechnology for Biofuels and Bioproducts, 17

[523] Engineering an Escherichia coli strain for enhanced production of flavonoids derived from pinocembrin (2024)

E. K. R. Hanko, C. J. Robinson, S. Bhanot, A. J. Jervis & N. S. Scrutton

Microbial Cell Factories, 23

DOI: doi.org/10.1186/s12934-024-02582-z

[522] Determinants of Product Outcome in Two Sesquiterpene Synthases from the Thermotolerant Bacterium Rubrobacter Radiotolerans (2024)

J. N. Whitehead, N. G. H. Leferink, S. Hay & N. S. Scrutton

ChemBioChem, 26, e202400672

DOI: 10.1002/cbic.202400672

[521] A growth-coupling strategy for improving the stability of terpenoid bioproduction in Escherichia coli (2024)

J. C. Tan, Q. Hu & N. S. Scrutton

Microb Cell Fact, 23

DOI: 10.1186/s12934-024-02548-1

[520] Engineering EPS in Halomonas bluephagenesis leads to self-flocculation and filamentation for convenient downstream processing (2024)

H. Park, L. Zhang, H. He, X. Yan, N. S. Scrutton & G-Q. Chen.

Chemical Engineering Journal, 495, 153374

DOI: 10.1016/j.cej.2024.153374

[519] A Long-Term Growth Stable Halomonas sp. Deleted with Multiple Transposases Guided by Its Metabolic Network Model Halo-ecGEM (2024)

L. Zhang, J-W. Ye, G. Li, H. Park, H. Luo, Y. Lin, S. Li, W. Yang, Y. Guan, F. Wu, W. Huang, Q. Wu, N. S. Scrutton, J. Nielson & G-Q. Chen

Metabolic Engineering, 84, 95-108

DOI:10.1016/j.ymben.2024.06.004

[518] Upgrading a cyanobacterial genome-scale model by inclusion of enzymatic constraints (2024)

F. Andrews, N. S. Scrutton & J-M. Schwartz

Algal Research, 81, 103584

DOI: 10.1016/j.algal.2024.103584

[517] Transitioning Enzyme Catalysis Towards Photocatalysis (2024)

D. J. Heyes, S. Hay & N. S. Scrutton

Phil. Trans. R. Soc, 383, 20230380

DOI: 10.1098/rsta.2023.0380

[516] Photocobilins integrate B12 and bilin photochemistry for enzyme control (2024)

S. Zhang, L. N. Jeffreys, H. Poddar, Y. Yu, C. Liu, K. Patel, L. O. Johannissen, L. Zhu, M. J. Cliff, C. Yan, G. Schiro, M. Weik, M. Sakuma, C. W. Levy, D. Leys, D. J. Heyes & N. S. Scrutton.

Nature Communications, 15

DOI: 10.1038/s41467-024-46995-1

[515] Ectoine hyperproduction by engineered Halomonas bluephagenesis (2024)

Q. Hu, S. Sun, Z. Zhang, W. Liu, X. Yi, H. He, N. S. Scrutton & G. Chen

Metabolic Engineering, 82, 238 - 249

DOI: 10.1016/j.ymben.2024.02.010

[514] PHA is not just a bioplastic! (2024)

P. Park, H. He, X. Yan, X. Liu, N. S. Scrutton & G. Chen

Biotechnology Advances, 71

DOI: 10.1016/j.biotechadv.2024.108320

[513] Non-Sterile Microbial Production of Chemicals based on Halomonas spp. (2024)

J. Zhang, X. Yan, H. Park, N. S. Scrutton, T. Chen & G. Chen

Current Opinion in Biotechnology, 85, 103064

DOI: 10.1016/j.copbio.2023.103064

[512] Mechanistic implications of the ternary complex structural models for the photoenzyme protochlorophyllide oxidoreductase (2023)

A. Taylor, S. Zhang, L. O. Johannissen, M. Sakuma, R. S. Phillips, A. P. Green, S. Hay, D. J. Heyes & N. S. Scrutton

FEBS Journal, 291, 1404-1421

DOI: 10.1111/febs.17025

Open Access

[511] Chemoautotrophic Production of Gaseous Hydrocarbons, Bioplastics and Osmolytes by a Novel Halomonas Species (2023)

M. Faulkner, R. Hoeven, P. P. Kelly, Y. Sun, H. Park, L. Liu, H. S. Toogood & N. S. Scrutton

Biotechnology for Biofuels and Bioproducts, 16

DOI: 10.1186/s13068-023-02404-1

Open Access

[510] Arabinose as an Overlooked Sugar for Microbial Bioproduction of Chemical Building Blocks (2023)

V. Kumar, D. Agrawal, R. Bommareddy, M. A. Islam, S. jacob, V. Balan, V. Singh, V. Thakur, N. Navani & N. S. Scrutton

Critical Reviews in Biotechnology, 44, 1103 - 1120

DOI: 10.1080/07388551.2023.2270702

[509] Decoding catalysis by terpene synthases (2023)

J. N. Whitehead, N. G. H. Leferink, L. O. Johannissen, S. Hay & N.S. Scrutton

ACS Catalysis, 13, 12774–12802

DOI: 10.1021/acscatal.3c03047

Open Access

[508] Mapping the Initial Stages of a Protective Pathway that Enhances Catalytic Turnover by a Lytic Polysaccharide Monooxygenase (2023)

J. Zhao, Y. Zhuo, D. E. Diaz, M. Shanmugam, A. J. Tefler, P. J. Linley, D. Kracher, T. Hayashi, L. S. Seibt, F. J. Hardy, O. Manners, T. M. Hedison, K. A. Hollywood, R. Spiess, K. M. Cain, S. Diaz-Moreno, N. S. Scrutton, M. Tovborg, P. H. Walton, D. J. Heyes, & A. P. Green

J. Am. Chem. Soc., 145, 20672–20682

DOI: 10.1021/jacs.3c06607

Open Access

[507] Redox driven B12-ligand switch drives CarH photoresponse (2023)

H. Poddar, R. Rios-Santacruz, D. J. Heyes, M. Shanmugam, A. Brookfield, L. O. Johannissen, C. W. Levy, L. N. Jeffreys, S. Zhang, M. Sakuma, J. P Colletier, S. Hay, G. Schiro, M. Weik, N. S. Scrutton & D. Leys

Nature Communications, 14

DOI:10.1038/s41467-023-40817-6

Open Access

[506] Structure-based design of small imine reductase panels for target substrates (2023)

Y. Yu, A. R. Casamajo, W. Finnigan, C. Schnepel, R. Barker, C. Morrill, R. S. Heath, L. De Maria, N. J. Turner & N. S. Scrutton

ACS Catalysis, 13, 12310–12321

DOI: 10.1021/acscatal.3c02278

Open Access

[505] Biosynthesis of cannabigerol and cannabigerolic acid, the gateways to further cannabinoid production. (2023)

L. Kearsey, C. Yan, N. Prandi, H. S. Toogood, E. Takano & N. S. Scrutton

Synthetic Biology, ysad010

DOI: 10.1093/synbio/ysad010

Open Access

[504] Unravelling the complexity of enzyme catalysis (2023)

N. S. Scrutton

FEBS J, 290, 2204-2207

DOI: 10.1111/febs.16796

[503] On-line Omics Platform Expedites Industrial Application of Halomonas bluephagenesis TD1.0 (2023)

H. Park, M. Faulkner, H. S. Toogood, G. Q. Chen & N. S. Scrutton

Bioinformatics and Biology Insights

Open Access

[502] Photoinduced Electron Transfer from a 1,4,5,6-Tetrahydro Nicotinamide Adenine Dinucleotide (Phosphate) Analog to Oxidized Flavin in an Ene-Reductase Flavoenzyme (2023)

M. Speirs, S. J. O. Hardman, A. I. Iorgu, L. O. Johannissen, D. J. Heyes, N. S. Scrutton, I. V. Sazanovich & S. Hay

J. Phys. Chem. Letts, 14, 3236 - 3242

DOI: 10.1021/acs.jpclett.3c00176

[501] High-titer Bio-Styrene Production Afforded by Whole-cell Cascade Biotransformation (2023)

H. L. Messiha, N. S. Scrutton & D. Leys

ChemCatChem, 15, e202201102

DOI: 10.1002/cctc.202201102

Open Access

[500] Mutation in a chlorophyll-binding motif of Brassica ferrochelatase enhances both heme and chlorophyll biosynthesis (2022)

M. Liu, W. Ma, X. Su, X. Zhang, Y. Lu, S. Zhang, J. Yan, D. Feng, L. Ma, A. Taylor, Y. Ge, Q. Cheng, K. Xu, Y. Wang, N. Li, A. Gu, J. Zhang, S. Luo, S. Xuan, X. Chen, N. S. Scrutton, C. Li, J. Zhao & S. Shen

Cell Reports, 41, 111758

DOI:10.1016/j.celrep.2022.111758

[499] StarCrete: a starch-based regolith biocomposite for extraterrestrial construction (2023)

A. Roberts & N. S. Scrutton

Open Engineering, 13, 20220390

DOI: 10.1515/eng-2022-0390

[498] The Mechanism of Action of Flavin-Dependent Halogenases (2022)

R. D. Barker, Y. Yu, L. De Maria, L. O. Johannissen & N. S. Scrutton

ACS Catalysis, 12, 15352–15360

DOI: 10.1021/acscatal.2c05231

[497] Editorial overview: Catalysis and regulation: The chemistry of catalysis (2022)

N. S. Scrutton & D. Galonić Fujimori

Current Opinion in Structural Biology, 77, 102499

DOI: 10.1016/j.sbi.2022.102499

[496] Catalysis by Nature's photoenzymes (2022)

A. Taylor, D. J. Heyes & N. S. Scrutton

Current Opinion in Structural Biology, 77,102491

DOI: 10.1016/j.sbi.2022.102491

Open Access

[495] Co-production of biofuel, bioplastics and biochemicals during extended fermentation of Halomonas bluephagenesis (2022)

H. Park, H. S. Toogood, G. Chen, & N. S. Scrutton

Microbial Biotech, 16, 307 - 321

DOI: 10.1111/1751-7915.14158

Open Access

[494] How a 10-epi-cubebol Synthase Avoids Premature Reaction Quenching to Form a Tricyclic Product at High Purity (2022)

J. N. Whitehead, N. G. H. Leferink, G. K. Ready, C. W. Levy, S. Hay, E. Takano & N. S. Scrutton

ACS Catalysis, 12, 12123 - 12131

DOI: 10.1021/acscatal.2c03155

Open Access

[493] Baseline proteomics characterisation of the emerging host biomanufacturing organism Halomonas bluephagenesis (2022)

M. Russell, A. Currin, W. Rowe, G. Cheng, P. Barran & N. S. Scrutton

Sci Data 9, 492

DOI: 10.1038/s41597-022-01610-0

Open Access

[492] Combined pulsed electron double resonance EPR and molecular dynamics investigations of calmodulin suggest effect of crowding agents on protein structure (2022)

A. M. Stewart, M. Shanmugam, R. J. Kutta, N. S. Scrutton, J. Lovett & S. Hay

Biochemistry, 61, 1735 - 1742

DOI: 10.1021/acs.biochem.2c00099

Open Access

[491] Solution-State Inter-Copper Distribution of Redox Partner-Linked Copper Nitrite Reductases: A Pulsed Electron-Electron Double Resonance Spectroscopy Study (2022)

T. M. Hedison, A. Iorgu, D. Calabrese, D. J. Heyes, M. Shanmugam & N. S Scrutton

J. Phys. Chem. Lett. 13, 6927 - 6934

DOI: 10.1021/acs.jpclett.2c01584

Open Access

[490] Single crystal spectroscopy and multiple structures from one crystal (MSOX) define catalysis in copper nitrite reductases (2022)

S. L. Rose, S. Baba, H. Okumura, S. V. Antonyuk, D. Sasaki, T. M. Hedison, M. Shanmugam, D. J. Heyes, N. S. Scrutton, T. Kumasaka, T. Tosha, R. R. Eady, M. Yamamoto & S. S. Hasnain

Proc. Natl. Acad. Sci. USA, 119, e2205664119

DOI: 10.1073/pnas.2205664119

[489] GeneORator: An Efficient Method for the Systematic Mutagenesis of Entire Genes (2022)

L. Green, N. S. Scrutton & A. Currin

Methods Mol Biol. 2461,111-122

DOI: 10.1007/978-1-0716-2152-3_7

[488] Bioproduction of Linalool from Paper Mill Waste (2022)

M. Rinaldi, S. Tait, H. S. Toogood & N. S. Scrutton

Frontiers in Bioengineering and Biotechnology

DOI: 10.3389/fbioe.2022.892896

Open Access

[487] How photoactivation triggers protochlorophyllide reduction: computational evidence of a stepwise hydride transfer during chlorophyll biosynthesis (2022)

L. O. Johannissen, A. Taylor, S. J. O. Hardman, D. J. Heyes, N. S. Scrutton & S. Hay

ACS Catalysis, 12, 4141 - 4148

DOI: 10.1021/acscatal.2c00866

[486] An unusual light-sensing function for coenzyme B12 in bacterial transcription regulator CarH (2022)

H. Poddar, D. J. Heyes, S. Zhang, S. J. Hardman, M. Sakuma & N. S. Scrutton

Methods in Enzymology, 668, 349 - 372

DOI: 10.1016/bs.mie.2021.12.010

[485] Molecular determinants of carbocation cyclisation in bacterial monoterpene synthases (2021)

N. G. H. Leferink, A. M. Escorcia, B. R. Ouwersloot, L. O. Johannissen, S. Hay, M. W. van der Kamp & N. S. Scrutton

ChemBioChem, 23, e202100688

DOI: 10.1002/cbic.202100688

Open Access

[484] Making molecules with photodecarboxylases: a great start or a false dawn? (2021)

T. M. Hedison, D. J. Heyes & N. S. Scrutton

Curr. Res. Chem. Biol, 2

DOI: 10.1016/j.crchbi.2021.100017

Open Access

[483] Combinatorial Use of Environmental Stresses and Genetic Engineering to Increase Ethanol Titres in Cyanobacteria (2021)

F. Andrews, M. Faulkner, H.S. Toogood & N. S. Scrutton

Biotechnology for Biofuels

DOI:10.1186/s13068-021-02091-w

Open Access

[482] Predictive engineering of class I terpene synthases using experimental and computational approaches (2021)

N. G. H. Leferink & N. S. Scrutton

ChemBioChem, 23, e202100484

DOI: 10.1002/cbic.202100484

Open Access

[481] Flavoprotein-dependent bioreductions. In Flavin-based catalysis: principles and applications (2021)

H. S. Toogood & N. S. Scrutton, (Eds., Cibulka & Fraaije), Wiley. Pp 201-224

DOI: 10.1002/9783527830138.ch8

[480] Blood, sweat and tears: extraterrestrial regolith biocomposites with in vivo binders (2021)

A. D. Roberts, D. R. Whittall, R. Breitling, E. Takanoa, J. J. Blaker, S. Hay and N. S. Scrutton

Materials Today Bio, 12, 100136

DOI: 10.1016/j.mtbio.2021.100136

Open Access

[479] Flavin oxidation state impacts on nitrofuran antibiotic binding orientation in nitroreductases (2021)

H. S. Toogood and N. S. Scrutton

Biochemical Journal, 478, 3423 - 3428

DOI: 10.1042/BCJ20210489

[478] Design and fabrication of recombinant reflectin-based multilayer reflectors: bio-design engineering and photoisomerism induced wavelength modulation (2021)

E. Wolde-Michael, A. D. Roberts, D. J. Heyes, A. G. Dumanli, J. J. Blaker, E. Takano, N. S. Scrutton

Scientific Reports,11, 14580

DOI: 10.1038/s41598-021-94042-6

Open Access

[477] Alternative metabolic pathways and strategies to high-titre terpenoid production in Escherichia coli (2021)

M. A. Rinaldi, C. A. Ferraz, and N. S. Scrutton

Natural Product Reports, 39, 90 - 118

DOI: 10.1039/D1NP00025J

Open Access

[476] Prototyping of microbial chassis for the biomanufacturing of high-value chemical targets (2021)

C. J. Robinson, J. Tellechea-Luzardo, P. Carbonell, A. J. Jervis, C. Yan, K. A. Hollywood, M. S. Dunstan, A. Currin, E. Takano, N. S. Scrutton

Biochem Soc Trans, BST20200017

DOI: 10.1042/BST20200017

[475] Enzyme immobilisation on wood-derived cellulose scaffolds via carbohydrate-binding module fusion constructs (2021)

A. D. Roberts, K. A. P. Payne, S. Cosgrove, V. Tilakaratna, I. Penafiel, W. Finnigan, N. Turner and N. S. Scrutton

Green Chemistry, 23, 4716, DOI:10.1039/D1GC01008E Open Access

[474] Consolidated bioprocessing: synthetic biology routes to fuels and fine chemicals (2021)

A. Banner, H. S. Toogood and N. S. Scrutton

Microorganisms, 9, 1079; , DOI:10.3390/microorganisms9051079

[473] Isopentenol utilization pathway for the production of linalool in Escherichia coli using an improved bacterial linalool/nerolidol synthase (2021)

C. A. Ferraz, N. G. H. Leferink, I. Kosov, and N. S. Scrutton

ChemBioChem, 22, 13, 2325 , DOI:10.1002/cbic.202100110 Open Access

[472] The evolving art of creating genetic diversity: from directed evolution to synthetic biology (2021)

A. Currin S. Parker, C. J. Robinson, E. Takano, N. S. Scrutton and R. Breitling

Biotech Advances, 50, 107762, DOI: 10.1016/j.biotechadv.2021.107762 Open Access

[471] A guide to time-resolved structural analysis of light-activated proteins (2021)

H. Poddar, D. J. Heyes, G. Schirò, M. Weik, D. Leys, N. S. Scrutton

FEBS Journal, In Press, DOI:10.1111/febs.15880 Open Access

[470] Cytochrome c fused copper nitrite reductases (2021)

T. M. Hedison and N. S. Scrutton

Encyclopedia of Inorganic and Bioinorganic Chemistry, DOI:10.1002/9781119951438.eibc2772

[469] Photocatalysis as the ‘master switch’ of photomorphogenesis in early plant development (2021)

D. J. Heyes, S. Zhang, A. Taylor, L. O. Johannissen, S. J. O. Hardman, S. Hay & N. S. Scrutton

Nature Plants , 7, 268, DOI: 10.1038/s41477-021-00866-5

[468] Inflammation control and improvement of cognitive function in COVID-19 infections: Is there a role for kynurenine 3-monooxygenase inhibition? (2021)

M. E. W. Collier, S. Zhang, N. S. Scrutton, F. Giorgini

Drug Discovery Today 26, 6, 1473, DOI: 10.1016/j.drudis.2021.02.009

[467] A plasmid toolset for CRISPR-mediated genome editing and CRISPRi gene regulation in Escherichia coli (2021)

A. J. Jervis, E. K.R. Hanko, M. S. Dunstan, C. J. Robinson, E. Takano , N. S. Scrutton

Microbial Biotechnology, 14, 3, 1120-1129, DOI: 10.1111/1751-7915.13780 Open Access

[466] Quantum biology: an update and perspective (2021)

Y. Kim, F. Bertagna, E. M. D’Souza, D. J. Heyes , L. O. Johannisse, E. T. Nery, A. Pantelias, A. Sanchez-Pedreño Jimenez, L. Slocombe, M. Spencer, J. Al-Khalili, G. S. Engel, S. Hay, S. M. Hingley-Wilson, K. Jeevaratnam, A. R. Jones, D. R. Kattnig, R. Lewis, M. Sacchi, N. S. Scrutton, S. R. P. Silva, J. McFadden

Quantum Reports, 3, 80-126 DOI:10.3390/quantum3010006 Open Access

[465] Insights into the H2O2-driven catalytic mechanism of fungal lytic polysaccharide monooxygenases (2021)

T. M. Hedison, E. Breslmayr, M. Shanmugam, K. Karnpakdee, D. J. Heyes, A. Green, R. Ludwig, N. S. Scrutton, D. Kracher

FEBS J, 288, 13, 4115, DOI: 10.1111/febs.15704 Open Access

[464] A biological route to conjugated alkenes: microbial production of hepta-1,3,5-triene (2021)

H. Messiha, K. Payne, N. S. Scrutton, D. Leys

ACS Synthetic Biology, 10, 228, DOI: 10.1021/acssynbio.0c00464 Open Access

[463] Advantages of brain penetrating inhibitors of kynurenine-3-monooxygenase for treatment of neurodegenerative diseases (2020)

S. Zhang, M. E.W. Collier, D. J. Heyes, F. Giorgini, N. S. Scrutton

Archives of Biochemistry and Biophysics, 697, 15, 108702, DOI: 10.1016/j.abb.2020.108702

[462] Streamlining natural products biomanufacturing with omics and machine learning-driven microbial engineering (2020)

A. B. Ramzi, S. N. Baharum, H. Bunawan, N. S. Scrutton

Front. Bioeng. Biotechnol., 8, 1412, DOI: 10.3389/fbioe.2020.608918

Open Access

[461] In conversation with Nigel Scrutton (2021)

P. Dillon and N. S. Scrutton

FEBS J, 28, 6, 1728-1733, DOI: 10.1111/febs.15632

[460] Engineering nature for gaseous hydrocarbon production (2020)

M. Amer, H. Toogood and N. S. Scrutton

Microbial Cell Factories, 19, 209, DOI: 10.1186/s12934-020-01470-6 Open Access

[459] Electron transfer flavoproteins (2020)

H. Toogood and N. S. Scrutton

Encyclopedia of Biophysics, DOI: 10.1007/978-3-642-35943-9_40-1

[458] Promoter engineering for microbial bio-alkane gas production (2020)

D. Trisrivirat, J. M. X. Hughes, R. Hoeven, M. Faulkner, H. Toogood, P.Chaiyen and N. S. Scrutton

Synthetic Biology, ysaa022, DOI: 10.1093/synbio/ysaa022 Open Access

[457] In silico design and automated learning to boost next-generation smart biomanufacturing (2020)

P. Carbonell, R. Le Feuvre, E. Takano, N. S. Scrutton

Synthetic Biology, ysaa020, DOI: 10.1093/synbio/ysaa020 Open Access

[456] Exploiting single domain antibodies as regulatory parts to modulate monoterpenoid production in E. coli (2020)

J. Wilkes, A. Scott-Tucker, M Wright, T. Crabbe, N.S. Scrutton

ACS Synth Biol., 9, 10, 2828–2839, DOI: 10.1021/acssynbio.0c00375 Open Access

[455] Dual role of the active site ‘lid’ regions of protochlorophyllide oxidoreductase in photocatalysis and plant development (2020)

S. Zhang, A.R.F. Godwin, A. Taylor, S.J.O. Hardman, T. A. Jowitt, L.O.Johannissen, S. Hay, C. Baldock, D.J. Heyes, N.S. Scrutton

FEBS J. 288, 1, 175-189, DOI: 10.1111/febs.15542 Open Access

[454] Production of the fragrance geraniol in peroxisomes of a product-tolerant baker’s yeast (2020)

J. Gerke, H. Frauendorf, D. Schneider, M. Wintergoller, T. Hofmeister, A. Poehlein, Z. Zebec, E. Takano, N.S. Scrutton, G. Braus

Front. Bioeng. Biotechnol., 8, 1097, DOI: 10.3389/fbioe.2020.582052

Open Access

[453] The catalytic mechanism of aromatic nitration by cytochrome P450 TxtE: involvement of a ferric-peroxynitrite intermediate (2020)

S. Louka, S. M. Barry, D. J. Heyes, M. Q. E. Mubarak, H. S. Ali, L. Alkhalaf, A. W. Munro, N. S. Scrutton, G. L. Challis, and S. P. de Visser

Journal of the American Chemical Society, 142, 15764–15779,

DOI: 10.1021/jacs.0c05070 Open Access

[452] Photocycle of cyanobacteriochrome TePixJ (2020)

S. J. O. Hardman, D. J. Heyes, I. V. Sazanovich, N. S. Scrutton

Biochemistry, 59, 2909–2915, DOI: 10.1021/acs.biochem.0c00382 Open Access

[451] Engineering Escherichia coli towards de novo production of gatekeeper (2S)-flavanones: naringenin, pinocembrin, eriodictyol and homoeriodictyol (2020)

M. S. Dunstan, C. J. Robinson, A. J. Jervis, C. Yan, P. Carbonell, K. A. Hollywood, A. Currin, N. Swainston, R. Le Feuvre, J. Micklefield, J.-L. Faulon, R. Breitling, N. Turner, E. Takano, N. S. Scrutton

Synthetic Biology, ysaa012, DOI: 10.1093/synbio/ysaa012 Open Access

[450] Renewable and tuneable bio-LPG blends derived from amino acids (2020)

M. Amer, R. Hoeven, P. Kelly, M. Faulkner, M. H. Smith, H. S. Toogood, and N. S. Scrutton

Biotechnology for Biofuels, 13, 125, DOI: 10.1186/s13068-020-01766-0 Open Access

[449] Non-covalent protein-based adhesives for transparent substrates: bovine serum albumin vs. recombinant spider silk (2020)

A. D. Roberts, W. Finnigan, P. P. Kelly, M. Faulkner, R. Breitling, E. Takano, N. S. Scrutton, J. J. Blaker and S. Hay

Materials Today Bio, 7, 100068, DOI: 10.1016/j.mtbio.2020.100068 Open Access

[448] The effect of terminal globular domains on the response of recombinant mini-spidroins to fiber spinning triggers (2020)

W. Finnigan, A. D. Roberts, C. Ligorio, N. S. Scrutton, R. Breitling, J. J. Blaker, E. Takano

Scientific Reports, 10, 10671, DOI: 10.1038/s41598-020-67703-1 Open Access

[447] Ultrafast vibrational energy transfer between protein and cofactor in a flavoenzyme (2020)

S. J. O. Hardman, A. I. Iorgu, D. J. Heyes, N. S. Scrutton, I. V. Sazanovich, S. Hay

Journal of Physical Chemistry B, 124, 5163-5168, DOI: 10.1021/acs.jpcb.0c04929

Open Access

[446] Photochemical mechanism of light-driven fatty acid photodecarboxylase (2020)

D. J. Heyes, B. Lakavath, S. J. O. Hardman, M. Sakuma, T. M. Hedison, N. S. Scrutton

ACS Catalysis, 10, 6691-6696, DOI: 10.1021/acscatal.0c01684 Open Access

[445] Thermal, electrochemical and photochemical reactions involving catalytically versatile ene reductase enzymes (2020)

H. S. Toogood, N. S. Scrutton

The Enzymes, Flavin Dependent Enzymes vol 47, 491 - 515, DOI: 10.1016/bs.enz.2020.05.012

[444] Active intermediates in copper nitrite reductase reactions probed by a cryotrapping-electron paramagnetic resonance approach (2020)

T. M. Hedison, M. Shanmugam, D. J. Heyes, R. Edge, N. S. Scrutton

Angewandte Chemie, 50, 13936-13940, DOI: 10.1002/anie.202005052 Open Access

[443] Flavin doesn’t put all oxygens in one basket (2020)

D. Leys and N. S. Scrutton

Nature Chemical Biology, 16, 485–486. DOI: 10.1038/s41589-020-0523-z

[442] Radical-based photoinactivation of fatty acid photodecarboxylases (2020)

B. Lakavath, T. M. Hedison, D. J. Heyes, M. Shanmugam, M. Sakuma, R. Hoeven, V. Tilakaratna, N. S. Scrutton

Analytical Biochemistry, 600, 113749, DOI: 10.1016/j.ab.2020.113749 Open Access

[441] Rapid prototyping of microbial production strains for the biomanufacture of potential materials monomers (2020)

C. J. Robinson, P. Carbonell, A. J. Jervis, C. Yan, K. A. Hollywood, M. S. Dunstan, A. Currin, N. Swainston, R. Spiess, S.Taylor, P. Mulherin, S. Parker, W. Rowe, N. E. Matthews, K. J. Malone, R. Le Feuvre, P.Shapira, P. Barran, N. J. Turner, J. Micklefield, R. Breitling, E. Takano, N. S. Scrutton

Metabolic Engineering, 60, 168-182, DOI: 10.1016/j.ymben.2020.04.008 Open Access

[440] Exploring novel bacterial terpene synthases (2020)

G. K Reddy, N. Leferink, M. Umemura, S. T. Ahmed, R. Breitling, N. S. Scrutton, E. Takano

PLOS ONE, 15(4): e0232220, DOI: 10.1371/journal.pone.0232220 Open Access

[439] Low carbon strategies for sustainable bio-alkane gas production and renewable energy (2020)

M. Amer, E. Z. Wojcik, C. Sun, R. Hoeven, J. M. X. Hughes, M. Faulkner, I. S. Yunus, S. Tait, L. O. Johannissen, S. J. O.Hardman, D. J. Heyes, G.-Q. Chen, M. H. Smith, P. R. Jones, H. S. Toogood & N. S. Scrutton

Energy & Environmental Science, 13, 1818-1831, DOI: 10.1039/D0EE00095G Open Access

[438] Protein conformational change is essential for reductive activation of lytic polysaccharide monooxygenase by cellobiose dehydrogenase (2020)

E. Breslmayr, C. Laurent, S. Scheiblbrandner,A. Jerkovic, D. J. Heyes, C. Oostenbrink, R. Ludwig, T. Hedison, N. S. Scrutton, D. Kracher

ACS Catalysis, 10, 4842-4853, DOI: 10.1021/acscatal.0c00754 Open Access

[437] Hierarchically porous silk/activated-carbon composite fibers for adsorption and repellence of volatile organic compounds (2020)

A. D. Roberts, J. M. Lee, A. Magaz, M. W. Smith, M. Dennis, N. S. Scrutton, J. J. Blaker

Molecules, 25, 1207, DOI: 10.3390/molecules25051207 Open Access

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