2020-2021 Publications

[482] Predictive engineering of class I terpene synthases using experimental and computational approaches (2021)

N. G. H. Leferink & N. S. Scrutton

ChemBioChem, Accepted

[481] Flavoprotein-dependent bioreductions. In Flavin-based catalysis: principles and applications (2021)

H. S. Toogood & N. S. Scrutton, (Eds., Cibulka & Fraaije), Wiley. Pp 201-224

DOI: 10.1002/9783527830138.ch8

[480] Blood, sweat and tears: extraterrestrial regolith biocomposites with in vivo binders (2021)

A. D. Roberts, D. R. Whittall, R. Breitling, E. Takanoa, J. J. Blaker, S. Hay and N. S. Scrutton

Materials Today Bio, 12, 100136, DOI: 10.1016/j.mtbio.2021.100136 Open Access

[479] Flavin oxidation state impacts on nitrofuran antibiotic binding orientation in nitroreductases (2021)

H. S. Toogood and N. S. Scrutton

Biochemical Journal, 478, 3423 DOI: 10.1042/BCJ20210489

[478] Design and fabrication of recombinant reflectin-based multilayer reflectors: bio-design engineering and photoisomerism induced wavelength modulation (2021)

E. Wolde-Michael, A. D. Roberts, D. J. Heyes, A. G. Dumanli, J. J. Blaker, E. Takano, N. S. Scrutton

Scientific Reports,11, 14580 DOI: 10.1038/s41598-021-94042-6 Open Access

[477] Alternative metabolic pathways and strategies to high-titre terpenoid production in Escherichia coli (2021)

M. A. Rinaldi, C. A. Ferraz, and N. S. Scrutton

Natural Product Reports, In press, DOI: 10.1039/D1NP00025J Open Access

[476] Prototyping of microbial chassis for the biomanufacturing of high-value chemical targets (2021)

C. J. Robinson, J. Tellechea-Luzardo, P. Carbonell, A. J. Jervis, C. Yan, K. A. Hollywood, M. S. Dunstan, A. Currin, E. Takano, N. S. Scrutton

Biochem Soc Trans, BST20200017, DOI: 10.1042/BST20200017

[475] Enzyme immobilisation on wood-derived cellulose scaffolds via carbohydrate-binding module fusion constructs (2021)

A. D. Roberts, K. A. P. Payne, S. Cosgrove, V. Tilakaratna, I. Penafiel, W. Finnigan, N. Turner and N. S. Scrutton

Green Chemistry, 23, 4716, DOI:10.1039/D1GC01008E Open Access

[474] Consolidated bioprocessing: synthetic biology routes to fuels and fine chemicals (2021)

A. Banner, H. S. Toogood and N. S. Scrutton

Microorganisms, 9, 1079; , DOI:10.3390/microorganisms9051079

[473] Isopentenol utilization pathway for the production of linalool in Escherichia coli using an improved bacterial linalool/nerolidol synthase (2021)

C. A. Ferraz, N. G. H. Leferink, I. Kosov, and N. S. Scrutton

ChemBioChem, 22, 13, 2325 , DOI:10.1002/cbic.202100110 Open Access

[472] The evolving art of creating genetic diversity: from directed evolution to synthetic biology (2021)

A. Currin S. Parker, C. J. Robinson, E. Takano, N. S. Scrutton and R. Breitling

Biotech Advances, 50, 107762, DOI: 10.1016/j.biotechadv.2021.107762 Open Access

[471] A guide to time-resolved structural analysis of light-activated proteins (2021)

H. Poddar, D. J. Heyes, G. Schirò, M. Weik, D. Leys, N. S. Scrutton

FEBS Journal, In Press, DOI:10.1111/febs.15880 Open Access

[470] Cytochrome c fused copper nitrite reductases (2021)

T. M. Hedison and N. S. Scrutton

Encyclopedia of Inorganic and Bioinorganic Chemistry, DOI:10.1002/9781119951438.eibc2772

[469] Photocatalysis as the ‘master switch’ of photomorphogenesis in early plant development (2021)

D. J. Heyes, S. Zhang, A. Taylor, L. O. Johannissen, S. J. O. Hardman, S. Hay & N. S. Scrutton

Nature Plants , 7, 268, DOI: 10.1038/s41477-021-00866-5

[468] Inflammation control and improvement of cognitive function in COVID-19 infections: Is there a role for kynurenine 3-monooxygenase inhibition? (2021)

M. E. W. Collier, S. Zhang, N. S. Scrutton, F. Giorgini

Drug Discovery Today 26, 6, 1473, DOI: 10.1016/j.drudis.2021.02.009

[467] A plasmid toolset for CRISPR-mediated genome editing and CRISPRi gene regulation in Escherichia coli (2021)

A. J. Jervis, E. K.R. Hanko, M. S. Dunstan, C. J. Robinson, E. Takano , N. S. Scrutton

Microbial Biotechnology, 14, 3, 1120-1129, DOI: 10.1111/1751-7915.13780 Open Access

[466] Quantum biology: an update and perspective (2021)

Y. Kim, F. Bertagna, E. M. D’Souza, D. J. Heyes , L. O. Johannisse, E. T. Nery, A. Pantelias, A. Sanchez-Pedreño Jimenez, L. Slocombe, M. Spencer, J. Al-Khalili, G. S. Engel, S. Hay, S. M. Hingley-Wilson, K. Jeevaratnam, A. R. Jones, D. R. Kattnig, R. Lewis, M. Sacchi, N. S. Scrutton, S. R. P. Silva, J. McFadden

Quantum Reports, 3, 80-126 DOI:10.3390/quantum3010006 Open Access

[465] Insights into the H2O2-driven catalytic mechanism of fungal lytic polysaccharide monooxygenases (2021)

T. M. Hedison, E. Breslmayr, M. Shanmugam, K. Karnpakdee, D. J. Heyes, A. Green, R. Ludwig, N. S. Scrutton, D. Kracher

FEBS J, 288, 13, 4115, DOI: 10.1111/febs.15704 Open Access

[464] A biological route to conjugated alkenes: microbial production of hepta-1,3,5-triene (2021)

H. Messiha, K. Payne, N. S. Scrutton, D. Leys

ACS Synthetic Biology, 10, 228, DOI: 10.1021/acssynbio.0c00464 Open Access

[463] Advantages of brain penetrating inhibitors of kynurenine-3-monooxygenase for treatment of neurodegenerative diseases (2020)

S. Zhang, M. E.W. Collier, D. J. Heyes, F. Giorgini, N. S. Scrutton

Archives of Biochemistry and Biophysics, 697, 15, 108702, DOI: 10.1016/j.abb.2020.108702

[462] Streamlining natural products biomanufacturing with omics and machine learning-driven microbial engineering (2020)

A. B. Ramzi, S. N. Baharum, H. Bunawan, N. S. Scrutton

Front. Bioeng. Biotechnol., 8, 1412, DOI: 10.3389/fbioe.2020.608918

Open Access

[461] In conversation with Nigel Scrutton (2021)

P. Dillon and N. S. Scrutton

FEBS J, 28, 6, 1728-1733, DOI: 10.1111/febs.15632

[460] Engineering nature for gaseous hydrocarbon production (2020)

M. Amer, H. Toogood and N. S. Scrutton

Microbial Cell Factories, 19, 209, DOI: 10.1186/s12934-020-01470-6 Open Access

[459] Electron transfer flavoproteins (2020)

H. Toogood and N. S. Scrutton

Encyclopedia of Biophysics, DOI: 10.1007/978-3-642-35943-9_40-1

[458] Promoter engineering for microbial bio-alkane gas production (2020)

D. Trisrivirat, J. M. X. Hughes, R. Hoeven, M. Faulkner, H. Toogood, P.Chaiyen and N. S. Scrutton

Synthetic Biology, ysaa022, DOI: 10.1093/synbio/ysaa022 Open Access

[457] In silico design and automated learning to boost next-generation smart biomanufacturing (2020)

P. Carbonell, R. Le Feuvre, E. Takano, N. S. Scrutton

Synthetic Biology, ysaa020, DOI: 10.1093/synbio/ysaa020 Open Access

[456] Exploiting single domain antibodies as regulatory parts to modulate monoterpenoid production in E. coli (2020)

J. Wilkes, A. Scott-Tucker, M Wright, T. Crabbe, N.S. Scrutton

ACS Synth Biol., 9, 10, 2828–2839, DOI: 10.1021/acssynbio.0c00375 Open Access

[455] Dual role of the active site ‘lid’ regions of protochlorophyllide oxidoreductase in photocatalysis and plant development (2020)

S. Zhang, A.R.F. Godwin, A. Taylor, S.J.O. Hardman, T. A. Jowitt, L.O.Johannissen, S. Hay, C. Baldock, D.J. Heyes, N.S. Scrutton

FEBS J. 288, 1, 175-189, DOI: 10.1111/febs.15542 Open Access

[454] Production of the fragrance geraniol in peroxisomes of a product-tolerant baker’s yeast (2020)

J. Gerke, H. Frauendorf, D. Schneider, M. Wintergoller, T. Hofmeister, A. Poehlein, Z. Zebec, E. Takano, N.S. Scrutton, G. Braus

Front. Bioeng. Biotechnol., 8, 1097, DOI: 10.3389/fbioe.2020.582052

Open Access

[453] The catalytic mechanism of aromatic nitration by cytochrome P450 TxtE: involvement of a ferric-peroxynitrite intermediate (2020)

S. Louka, S. M. Barry, D. J. Heyes, M. Q. E. Mubarak, H. S. Ali, L. Alkhalaf, A. W. Munro, N. S. Scrutton, G. L. Challis, and S. P. de Visser

Journal of the American Chemical Society, 142, 15764–15779,

DOI: 10.1021/jacs.0c05070 Open Access

[452] Photocycle of cyanobacteriochrome TePixJ (2020)

S. J. O. Hardman, D. J. Heyes, I. V. Sazanovich, N. S. Scrutton

Biochemistry, 59, 2909–2915, DOI: 10.1021/acs.biochem.0c00382 Open Access

[451] Engineering Escherichia coli towards de novo production of gatekeeper (2S)-flavanones: naringenin, pinocembrin, eriodictyol and homoeriodictyol (2020)

M. S. Dunstan, C. J. Robinson, A. J. Jervis, C. Yan, P. Carbonell, K. A. Hollywood, A. Currin, N. Swainston, R. Le Feuvre, J. Micklefield, J.-L. Faulon, R. Breitling, N. Turner, E. Takano, N. S. Scrutton

Synthetic Biology, ysaa012, DOI: 10.1093/synbio/ysaa012 Open Access

[450] Renewable and tuneable bio-LPG blends derived from amino acids (2020)

M. Amer, R. Hoeven, P. Kelly, M. Faulkner, M. H. Smith, H. S. Toogood, and N. S. Scrutton

Biotechnology for Biofuels, 13, 125, DOI: 10.1186/s13068-020-01766-0 Open Access

[449] Non-covalent protein-based adhesives for transparent substrates: bovine serum albumin vs. recombinant spider silk (2020)

A. D. Roberts, W. Finnigan, P. P. Kelly, M. Faulkner, R. Breitling, E. Takano, N. S. Scrutton, J. J. Blaker and S. Hay

Materials Today Bio, 7, 100068, DOI: 10.1016/j.mtbio.2020.100068 Open Access

[448] The effect of terminal globular domains on the response of recombinant mini-spidroins to fiber spinning triggers (2020)

W. Finnigan, A. D. Roberts, C. Ligorio, N. S. Scrutton, R. Breitling, J. J. Blaker, E. Takano

Scientific Reports, 10, 10671, DOI: 10.1038/s41598-020-67703-1 Open Access

[447] Ultrafast vibrational energy transfer between protein and cofactor in a flavoenzyme (2020)

S. J. O. Hardman, A. I. Iorgu, D. J. Heyes, N. S. Scrutton, I. V. Sazanovich, S. Hay

Journal of Physical Chemistry B, 124, 5163-5168, DOI: 10.1021/acs.jpcb.0c04929

Open Access

[446] Photochemical mechanism of light-driven fatty acid photodecarboxylase (2020)

D. J. Heyes, B. Lakavath, S. J. O. Hardman, M. Sakuma, T. M. Hedison, N. S. Scrutton

ACS Catalysis, 10, 6691-6696, DOI: 10.1021/acscatal.0c01684 Open Access

[445] Thermal, electrochemical and photochemical reactions involving catalytically versatile ene reductase enzymes (2020)

H. S. Toogood, N. S. Scrutton

The Enzymes, Flavin Dependent Enzymes vol 47, 491 - 515, DOI: 10.1016/bs.enz.2020.05.012

[444] Active intermediates in copper nitrite reductase reactions probed by a cryotrapping-electron paramagnetic resonance approach (2020)

T. M. Hedison, M. Shanmugam, D. J. Heyes, R. Edge, N. S. Scrutton

Angewandte Chemie, 50, 13936-13940, DOI: 10.1002/anie.202005052 Open Access

[443] Flavin doesn’t put all oxygens in one basket (2020)

D. Leys and N. S. Scrutton

Nature Chemical Biology, 16, 485–486. DOI: 10.1038/s41589-020-0523-z

[442] Radical-based photoinactivation of fatty acid photodecarboxylases (2020)

B. Lakavath, T. M. Hedison, D. J. Heyes, M. Shanmugam, M. Sakuma, R. Hoeven, V. Tilakaratna, N. S. Scrutton

Analytical Biochemistry, 600, 113749, DOI: 10.1016/j.ab.2020.113749 Open Access

[441] Rapid prototyping of microbial production strains for the biomanufacture of potential materials monomers (2020)

C. J. Robinson, P. Carbonell, A. J. Jervis, C. Yan, K. A. Hollywood, M. S. Dunstan, A. Currin, N. Swainston, R. Spiess, S.Taylor, P. Mulherin, S. Parker, W. Rowe, N. E. Matthews, K. J. Malone, R. Le Feuvre, P.Shapira, P. Barran, N. J. Turner, J. Micklefield, R. Breitling, E. Takano, N. S. Scrutton

Metabolic Engineering, 60, 168-182, DOI: 10.1016/j.ymben.2020.04.008 Open Access

[440] Exploring novel bacterial terpene synthases (2020)

G. K Reddy, N. Leferink, M. Umemura, S. T. Ahmed, R. Breitling, N. S. Scrutton, E. Takano

PLOS ONE, 15(4): e0232220, DOI: 10.1371/journal.pone.0232220 Open Access

[439] Low carbon strategies for sustainable bio-alkane gas production and renewable energy (2020)

M. Amer, E. Z. Wojcik, C. Sun, R. Hoeven, J. M. X. Hughes, M. Faulkner, I. S. Yunus, S. Tait, L. O. Johannissen, S. J. O.Hardman, D. J. Heyes, G.-Q. Chen, M. H. Smith, P. R. Jones, H. S. Toogood & N. S. Scrutton

Energy & Environmental Science, 13, 1818-1831, DOI: 10.1039/D0EE00095G Open Access

[438] Protein conformational change is essential for reductive activation of lytic polysaccharide monooxygenase by cellobiose dehydrogenase (2020)

E. Breslmayr, C. Laurent, S. Scheiblbrandner,A. Jerkovic, D. J. Heyes, C. Oostenbrink, R. Ludwig, T. Hedison, N. S. Scrutton, D. Kracher

ACS Catalysis, 10, 4842-4853, DOI: 10.1021/acscatal.0c00754 Open Access

[437] Hierarchically porous silk/activated-carbon composite fibers for adsorption and repellence of volatile organic compounds (2020)

A. D. Roberts, J. M. Lee, A. Magaz, M. W. Smith, M. Dennis, N. S. Scrutton, J. J. Blaker

Molecules, 25, 1207, DOI: 10.3390/molecules25051207 Open Access