2015 - 2019 Publications

[435] Taming the reactivity of monoterpene synthases to guide regioselective product hydroxylation (2019)

N. G. H. Leferink, K. E. Ranaghan, J. Battye, L. O. Johannissen, S. Hay, M. W. van der Kamp, A. J. Mulholland, and N. S. Scrutton

ChemBioChem, In Press, DOI: 10.1002/cbic.201900672 Open Access

[434] Observation of the Δg mechanism resulting from the ultrafast spin dynamics that follow the photolysis of coenzyme B12 (2019)

J. A. Hughes, S. J. O. Hardman, N.S. Scrutton, D. M. Graham, J. R. Woodward, and A. R. Jones

Journal of Chemical Physics, 151, 201102, DOI: 10.1063/1.5127258

[433] Structural basis for enzymatic photocatalysis in chlorophyll biosynthesis (2019)

S. Zhang, D. J. Heyes, L. Feng, W. Sun, L. O. Johannissen, H. Liu, C. W. Levy, X. Li, J. Yang, X. Yu, M. Lin, S. J. O. Hardman, R. Hoeven, M. Sakuma, S. Hay, D. Leys, Z. Rao, A. Zhou, Q. Cheng & N. S. Scrutton

Nature, 574, 722-725, DOI: 10.1038/s41586-019-1685-2

[432] Structure of the Cannabis sativa olivetol-producing enzyme reveals cyclization plasticity in Type III polyketide synthases (2019)

L. J. Kearsey, N. Prandi, V. Karuppiah, C. Yan, D. Leys, H. Toogood, E. Takano and N. S. Scrutton

FEBS J, In Press, DOI: 10.1111/febs.15089 Open Access

[431] Highly multiplexed, fast and accurate nanopore sequencing for verification of synthetic DNA constructs and sequence libraries (2019)

A. Currin, N. Swainston, M. Dunstan, A. J. Jervis, P.Mulherin, C. J. Robinson,S. Taylor, P. Carbonell, K. A. Hollywood, C. Yan, E. Takano, N. S. Scrutton, R. Breitling.

Synthetic Biology, In Press, DOI: 10.1093/synbio/ysz025 Open Access

[430] Graphene–aramid nanocomposite fibres via superacid co-processing (2019)

A. D. Roberts, P. Kelly, J. Bain, J. J. Morrison, I. Wimpenny, M. Barrow, R. T. Woodwarde, M. Gresil, C. Blanford, S. Hay, J. J. Blaker, S. G. Yeates and N. S. Scrutton

Chemical Communications, 55, 11703-11706, DOI: 10.1039/C9CC04548A Open Access

[429] An automated pipeline for the screening of diverse monoterpene synthase libraries (2019)

N. G. H.Leferink, M. S. Dunstan, K. A. Hollywood, N. Swainston, A. Currin, A. J. Jervis, E. Takano & N. S. Scrutton

Scientific Reports, 9, 11936, DOI: 10.1038/s41598-019-48452-2 Open Access

[428] A brain-permeable inhibitor of the neurodegenerative disease target kynurenine 3-monooxygenase prevents accumulation of neurotoxic metabolites (2019)

S. Zhang, M. Sakuma, G. S. Deora, C. W. Levy, A. Klausing, C. Breda, K. D. Read, C. D. Edlin, B. P. Ross, M. Wright Muelas, P. J. Day, S. O’Hagan, D. B. Kell, R. Schwarcz, D. Leys, D. J. Heyes, F. Giorgini & N. S. Scrutton

Communications Biology, 2, 271, DOI:10.1038/s42003-019-0520-5 Open Access

[427] Unexpected roles of a tether harboring a tyrosine gatekeeper residue in modular nitrite reductase catalysis (2019)

T. M. Hedison, R. T. Shenoy, A. I. Iorgu, D. J. Heyes, K. Fisher, G. Wright, S. Hay, R. R. Eady, S. Antonyuk, S. S. Hasnain, N. S. Scrutton

ACS Catalysis, 9, 7, 6087-6099, DOI: 10.1021/acscatal.9b01266 Open Access

[426] The photochemical spin dynamics of the vitamin B12 derivative methylcobalamin (2019)

V.Lukinović, J. R. Woodward, T. C. Marrafa, M. Shanmugam, D. J. Heyes, S. J. O. Hardman, N. S. Scrutton, S. Hay, A. J. Fielding and A. R. Jones

Journal of Physical Chemistry B, 123, 22, 4663-4672, DOI: 10.1021/acs.jpcb.9b01969

[425] Building a global alliance of biofoundries (2019)

N. Hillson, M. Caddick, Y. Cai, J.A. Carrasco, M. W. Chang, N. C. Curach, D. J. Bell, R. Le Feuvre, D. C. Friedman, X. Fu, N. D. Gold, M. J. Herrgård, M.B. Holowko, J. R. Johnson, R. A. Johnson, J. D. Keasling, R. I. Kitney, A. Kondo, C. Liu, V. J. J. Martin, F. Menolascina, C. Ogino, N. J. Patron, M. Pavan, C. L. Poh, I. S. Pretorius, S. J. Rosser, N. S. Scrutton, M. Storch, H. Tekotte, E. Travnik, C. E. Vickers, W. S. Yew, Y. Yuan, H. Zhao & P. S. Freemont

Nature Communications, 10, 2040. DOI:10.1038/s41467-019-10079-2 Open Access

[424] What are the signatures of tunnelling in enzyme-catalysed reactions? (2019)

L. O. Johannissen, A. I. Iorgu, N. S. Scrutton, and S. Hay

Faraday Discussions, In Press, DOI: 10.1039/C9FD00044E

[423] Selectivity through discriminatory induced fit enables switching of NAD(P)H coenzyme specificity in Old Yellow Enzyme ene-reductases (2019)

A. I. Iorgu, T. M. Hedison, S. Hay and N. S. Scrutton

FEBS J, 286, 16, 3117-3128, DOI: 10.1111/febs.14862 Open Access

[422] Equatorial active site compaction and electrostatic reorganization in catechol-O-methyltransferase (2019)

S. Czarnota, L. O. Johannissen, N. J. Baxter, F. Rummel, A.Wilson, M. J. Cliff, C. W. Levy, N. S. Scrutton, J.P. Waltho, and S. Hay

ACS Catalysis, 9, 4394-4401 DOI: 10.1021/acscatal.9b00174 Open Access

[421] Isotopically labeled flavoenzymes and their uses in probing reaction mechanisms (2019)

A. I. Iorgu, M. J. Cliff, J. P. Waltho, N. S. Scrutton and S. Hay

Methods in Enzymology, 620, 145-166, DOI: 10.1016/bs.mie.2019.03.009

[420] SelProm: a queryable and predictive expression vector selection tool for Escherichia coli (2019)

A. J. Jervis, P. Carbonell, S. Taylor, R. Sung, M. S. Dunstan , C. J. Robinson, R. Breitling, E. Takano and N. S. Scrutton

ACS Synthetic Biology, 8, 7, 1478-1483, DOI: 10.1021/acssynbio.8b00399 Open Access

[419] Synthetic biology for fibres, adhesives and active camouflage materials in protection and aerospace (2019)

A. D. Roberts, W. Finnigan, E. Wolde-Michael, P. Kelly, J. J. Blaker, S. Hay, R. Breitling, E. Takano and N. S. Scrutton

MRS Communications, 9, 2, 486-504, DOI: 10.1557/mrc.2019.35 Open Access

[418] Solvent-slaved protein motions accompany proton coupled electron transfer reactions catalysed by copper nitrite reductase (2019)

T. M. Hedison, D. J. Heyes, M. Shanmugam, A. I. Iorgu and N. S. Scrutton

Chemical Communications, 55, 5863-5866, DOI: 10.1039/C9CC01026B Open Access

[417] Chemo-enzymatic routes towards the synthesis of bio-based monomers and polymers. (2019)

S. T. Ahmed, N. G. H. Leferink and N. S. Scrutton

Molecular Catalysis, 467, 95-110 DOI: 10.1016/j.mcat.2019.01.036

[416] Tripping the light fantastic in membrane redox biology: linking dynamic structures to function in P450 electron transfer chains (2019)

T. M. Hedison, N. S. Scrutton

FEBS Journal, 286, 11, 2004-2017, DOI: 10.1111/febs.14757 Open Access

[415] Light-induced structural changes in a full-length cyanobacterial phytochrome probed by time-resolved X-ray scattering (2019)

D. J. Heyes, S. J. O. Hardman, M. N. Pedersen, J. Woodhouse, E. De La Mora, M. Wulff, M. Weik, M.Cammarata, N. S. Scrutton & G. Schirò

Communications Biology, 2:1, DOI: 10.1038/s42003-018-0242-0 Open Access

[414] Machine learning of designed translational control allows predictive pathway optimisation in Escherichia coli (2019)

A.J. Jervis, P. Carbonell, M. Vinaixa, M. Dunstan, K. Hollywood, C. J. Robinson, N. Rattray, C. Yan, N. Swainston, A. Currin, R. Sung, H. S. Toogood, S. Taylor, J-L. Faulon, R. Breitling, E. Takano, N. S. Scrutton

ACS Synthetic Biology, 8, 127–136, DOI: 10.1021/acssynbio.8b00398 Open Access

[413] C3 and C6 modification-specific OYE biotransformations of synthetic carvones and sequential BVMO chemoenzymatic synthesis of chiral caprolactones (2019)

I. S. Issa, H. S. Toogood, L. O. Johannissen, J. Raftery, N. S. Scrutton & J. M. Gardiner

Chemistry: A European Journal, 25, 2983-2988, DOI: 10.1002/chem.201805219 Open Access

[412] Preface - Methods in Enzymology (2018)

N. S. Scrutton

Methods in Enzymology, Volume 608. DOI:10.1016/S0076-6879(18)30341-0

[411] From bugs to bioplastics: Total (+)-dihydrocarvide biosynthesis by engineered Escherichia coli (2018)

G.A. Ascue Avalos, H. S. Toogood, S. Tait, H. L. Messiha and N. S. Scrutton

ChemBioChem, 20, 6, 785-792, DOI: 10.1002/cbic.201800606

[410] Nonequivalence of second sphere 'noncatalytic' residues in pentaerythritol tetranitrate reductase in relation to local dynamics linked to H-transfer reactions with NADH and NADPH coenzymes (2018)

A. I. Iorgu, N. J. Baxter, M. J. Cliff, C. W. Levy, J. P. Waltho, S. Hay, N. S. Scrutton.

ACS Catalysis, 8, 11589-11599, DOI: 10.1021/acscatal.8b02810 Open Access

[409] Selective cellular imaging with lanthanide based upconversion nanoparticles (2018)

P.P. Nampi, A. Vakurov, L.E. Mackenzie, N.S. Scrutton, P.A. Millner, G. Jose, S. Saha.

Journal of Biophotonics. 12, 4, e201800256, DOI: 10.1002/jbio.201800256 Open Access

[408] The sacrificial inactivation of the blue-light photosensor cryptochrome from Drosophila melanogaster (2018)

R.J. Kutta, N. Archipowa, N.S. Scrutton.

Physical Chemistry Chemical Physics, 20, 28767-28776, DOI: 10.1039/C8CP04671A Open Access

[407] A toolbox for diverse oxyfunctionalisation of monoterpenes (2018)

A. Hernandez-Ortega, M. Vinaixa, Z. Zebec, E. Takano, N. S. Scrutton.

Scientific Reports 8, 14396, DOI: 10.1038/ s41598-018-32816-1 Open Access

[406] A biocatalytic method for the chemoselective aerobic oxidation of aldehydes to carboxylic acids (2018)

T. Knaus, V. Tseliou, L.D. Humphreys, N. S. Scrutton, F. G. Mutti.

Green Chemistry 20, 3931-3943, DOI: 10/1039/C8GC01381K Featured on front cover

[405] Chemoenzymatic synthesis of the intermediates in the peppermint monoterpenoid biosynthetic pathway (2018)

A. Ni Cheallaigh, H. S. Toogood, D. J. Mansell, A. Lygidakis, S. Tait, N. S. Scrutton, J. M. Gardiner.

Journal of Natural Products. 81, 1546-1552, DOI: 10.1021/acs.jnatprod.7b01026.

[404] An automated Design-Build-Test-Learn pipeline for enhanced microbial production of fine chemicals (2018)

P. Carbonell, A. J. Jervis, C. J. Robinson, C. Yan, M. Dunstan, N. Swainston, M. M. Vinaixa, K. A. Hollywood, A. Currin, N. J. W. Rattray, S. Taylor, R. Spiess, R. Sung, A. R. Williams, D. Fellows, N. J. Stanford, P. Mulherin, R. Le Feuvre, P. Barran, R. Goodacre, N. J. Turner, C. Goble, G. G. Chen, D. B. Kell, J. Micklefield, R. Breitling, E. Takano, J. -L. Faulon, N. S. Scrutton

Communications Biology 1, 66. DOI: 10.1038/s42003-018-0076-9 Open Access

[403] Genome editing for the production of natural products in Escherichia coli (2018).

Z. Zebec & N. S. Scrutton

Advanced Biosystems. 2, 11, 1800056, DOI: 10.1002/adbi.201800056

[402] Multi-fragment DNA assembly of biochemical pathways via automated ligase cycling reaction (2018).

C.J. Robinson, M.S. Dunston, N. Swainston, J. Titchmarsh, E. Takano, N.S. Scrutton, A.J. Jervis,

Methods in Enzymology. 608, 369-392, DOI: 10.1016/bs.mie.2018.04.011

[401] A living foundry for synthetic biological materials: a synthetic biology roadmap to new advanced materials (2018)

R. Le Feuvre, N.S. Scrutton,

Synthetic and Systems Biotechnology. 3, 105-112, DOI: 10.1016/j.synbio.2018.04.002 Open Access

[400] Experiment and simulation reveal how mutations in functional plasticity regions guide plant monoterpene synthase product outcome (2018)

N.G.H. Leferink, K.E. Ranaghan, V. Karuppiah, A. Currin, M.W. van der Kamp, A.J. Mulholland, N.S. Scrutton,

ACS Catalysis 8, 3780-3791, DOI: 10.1021/acscatal.8b00692

[399] Discovery, characterisation, engineering and applications of ene reductases for industrial biocatalysis (2018)

H.S. Toogood, N. S. Scrutton,

ACS Catalysis, 8, 3532-3549 DOI: 10.1021/acscatal.8b00624

[398] Photochemical mechanism of an atypical algal phytochrome (2018)

U.Choudry, D.J. Heyes, S.J.O. Hardman, M. Sakuma, I.V. Sazanovich, J. Woodhouse, E. De La Mora, M. Pedersen, M. Wulff, M. Weik, G. Schiro, N.S. Scrutton,

ChemBioChem, 19, 1036-1043, DOI: 10.1002/cbic.201800016 . Featured on front cover

[397] Biocatalytic routes to lactone monomers for sustainable polymer production (2018)

H.L. Messiha, S.T. Ahmed, V. Karuppiah, R. Suardiaz, G.A. Avalos, N. Fey, S. Yeates, H.S. Toogood, A.J. Mulholland, N. S. Scrutton,

Biochemistry, 57, 1997-2008 DOI: 10.1021/acs.biochem.8b00169

[396] PartsGenie: an integrated tool for optimising and sharing synthetic biology parts (2018)

N. Swainston, M. Dunstan, A. J. Jervis, C. J. Robinson, P. Carbonell, A. R. Williams, J-L Faulon, N. S. Scrutton, D. B. Kell.

Bioinformatics, 34, 13, 2327–2329, DOI: 10.1093/bioinformatics/bty105

[395] Selenzyme: enzyme selection tool for pathway design (2018)

P. Carbonell, J. Wong, N.Swainston, E. Takano, N.J. Turner, N.S. Scrutton, D.B. Kell, R. Breitling, J-L. Faulon,

Bioinformatics, 34, 12, 2153–2154, DOI: 10.1093/bioinformatics/bty065, Open Access

[394] Engineering the ‘missing link’ in biosynthetic (–)-menthol production: bacterial isopulegone isomerase (2018)

A. Currin, M. Dunston, L. Johannissen, K. Hollywood, M. Vinaixa, A. Jervis, N. Swainston, N. Rattray, J. Gardiner, D.B. Kell, E. Takano, H. S. Toogood, N. S. Scrutton,

ACS Catalysis, 8, 2012-2020 DOI: 10.1021/acscatal.7b04115 Open Access

[393] Stepwise hydride transfer in a biological system: insights into the reaction mechanism of the light-dependent protochlorophyllide oxidoreductase (2018)

N. Archipowa, R.J. Kutta, D.J. Heyes and N.S. Scrutton,

Angewandte Chemie, 57, 10, 2682-2686, DOI:10.1002/anie.201712729 Open Access

[392] Trapping methods for probing functional intermediates in nitric oxide synthases and related enzymes (2018)

T. M. Hedison, S. Hay, N. S. Scrutton

Frontiers in Bioscience, Landmark, 23, 1874-1888, DOI:10.2741/4678

[391] Bio-derived production of cinnamyl alcohol via a three step biocatalytic cascade and metabolic engineering (2018)

E. Klumbys, Z. Ziga, N. Weise, N. J. Turner, N. S. Scrutton

Green Chemistry, 20, 658-663 DOI: 10.1039/C7GC03325G

[390] 1H, 15N and 13C backbone resonance assignments of pentaerythritol tetranitrate reductase from Enterobacter cloacae PB2 (2017)

A. I. Iorgu, N. J. Baxter, M. J. Cliff, J. P. Waltho, S. Hay, N. S. Scrutton

Biomolecular NMR Assignments 12(1), 79-83, DOI: 10.1007/s12104-017-9791-2 Open Access

[389] Ab initio QM/MM modelling of the rate-limiting proton transfer step in the deamination of tryptamine by aromatic amine dehydrogenase (2017)

K. Ranaghan, W. Morris, L. Masgrau, K. Senthilkumar, L. Johannissen, N. S. Scrutton, J. Harvey, F. Manby, A. J. Mulholland

Journal of Physical Chemistry B, 121, 9785-9798, DOI: 10.1021/acs.jpcb.7b06892

[388] Enzymes make light work of hydrocarbon production (2017)

N. S. Scrutton

Science 357, 872-873, DOI: 10.1126/science.aao4399

[387] Retooling microorganisms for the fermentative production of alcohols (2017)

H. S. Toogood and N. S. Scrutton

Current Opinion in Biotechnology, 50, 1-10, DOI: 10.1016/j.copbio.2017.08.010 Open Access

[386] Structural basis of catalysis in the bacterial monoterpene synthases linalool synthase and 1,8-cineole synthase (2017)

V. Karuppiah, K. Ranaghan, N. G.H. Leferink, L. O. Johannissen, M. Shanmugam, A. Ni Cheallaigh, N. Bennett, L. Kearsey, E. Takano, J. Gardiner, M. Van der Kamp, S. Hay, A. J. Mulholland, D. Leys, and N. S. Scrutton

ACS Catalysis, 7(9), 6268-6282, DOI: 10.1021/acscatal.7b01924 Open Access

[385] Structures of carboxylic acid reductase reveal domain dynamics underlying catalysis (2017)

D. Gahloth, M. S. Dunstan, D. Quaglia, E. Klumbys, M. P. Lockhart-Cairns, A. M. Hill, S. R. Derrington, N. S. Scrutton, N. J. Turner & D. Leys

Nature Chemical Biology,13, 975-981 DOI:10.1038/nchembio.2434

[384] Liver microsomal lipid enhances the activity and redox coupling of co-localised cytochrome P450 reductase-cytochrome P450 3A4 in nanodiscs (2017)

K-C Liu, J. X. Hughes, S. Hay, N. S. Scrutton

FEBS Journal, 284, 14, 2303-2319, DOI: 10.1111/febs.14129 Open Access

[383] biochem4j: integrated and extensible biochemical knowledge through graph databases (2017)

N. Swainston, R. Batista-Navarro, P. Carbonell, P. D. Dobson, M. Dunstan, A. J. Jervis, M. Vinaixa, A. R. Williams, S. Ananiadou, J-L. Faulon, P. Mendes, D. B. Kell, N. S. Scrutton, R. Breitling

PloS One, 12(7): e0179130, DOI: 10.1371/journal.pone.0179130 Open Access

[382] Structural insights into the ene-reductase synthesis of profens (2017)

J. Waller, H. S. Toogood, V. Karuppiah, N. Rattray, D. J. Mansell, D. Leys, J. M. Gardiner, A. Fryszkowska, S. T. Ahmed, R. Bandichhor, G. P. Reddy, N. S. Scrutton

Organic & Biomolecular Chemistry, 15, 4440-4448 , DOI: 10.1039/C7OB00163K Open Access

[381] Convergence of theory and experiment on the role of preorganization, quantum tunneling and enzyme motions into flavoenzyme-catalyzed hydride transfer (2017)

M. Delgado, S. Görlich, J. E. Longbotham, N. S. Scrutton, S. Hay, V. Moliner, I. Tuñón

ACS Catalysis, 7, 3190-3198, DOI: 10.1021/acscatal.7b00201

[380] Vertebrate cryptochromes are vestigial flavoproteins (2017)

R. J. Kutta, N. Archipowa, L. O. Johannissen, A.R. Jones, N. S. Scrutton

Scientific Reports, 7, 44906, DOI: 10.1038/srep44906 Open Access

[379] Direct evidence of an excited state triplet species upon photoactivation of the chlorophyll precursor protochlorophyllide (2017)

G. Brandariz-de-Pedro, D.J. Heyes, S.J.O. Hardman, M.Shanmugam, A.R. Jones, S. Weber, D. Nohr, N.S. Scrutton, A.J. Fielding

Journal of Physical Chemistry Letters, 8 (6), 1219–1223, DOI: 10.1021/acs.jpclett.7b00200

[378] Excited state properties of protochlorophyllide analogues and implications for light driven synthesis of chlorophyll (2017)

Heyes, D. J., Hardman, S. J. O., Mansell, D., Ní Cheallaigh, A., Gardiner, J. M., Johannissen, L. O., Greetham, G. M., Towrie, M., Scrutton, N. S.,

Journal of Physical Chemistry B., 121, 1312-1320, DOI: 10.1021/acs.jpcb.7b00528

[377] Speeding up enzyme engineering computationally (2017)

Scrutton, N. S.

IUCrJ 4, 5-6 DOI:10.1107/S2052252516019692 Open Access

[376] 1H, 15N, 13C backbone resonance assignments of human soluble catechol O-methyltransferase in complex with S-adenosyl-l-methionine and 3,5-dinitrocatechol (2016)

Czarnota, S., Baxter, N. J., Cliff, M. J., Waltho, J. P., Scrutton, N. S., Hay, S.

Biomolecular NMR Assignments, DOI: 10.1007/s12104-016-9720-9 Open Access

[375] An oxidative N-demethylase reveals PAS transition from ubiquitous sensor to enzyme (2016)

Ortmayer, M., Lafite, P., Menon, B. R. K., Tralau, T., Fisher, K., Denkhaus, L., Scrutton, N. S., Rigby, S. E. J., Munro, A. W., Hay, S., Leys, D.

Nature, 539, 593-597, DOI: 10.1038/nature20159

[374] Engineering proximal vs distal heme-NO coordination via dinitrosyl dynamics: implications for NO sensor design (2017)

Kekilli, D., Petersen, C. A., Pixton, D. A., Ghafoor, D. D.,Abdullah, G. H., Dworkowski, F. S. N. Wilson, M. T., Heyes, D. J., Hardman, S. J. O., Murphy, L. M., Strange, R. W., Scrutton, N. S.,Andrew, C. R., Hough, M. A.

Chemical Science, 8, 1986-1994; DOI: 10.1039/C6SC04190F Open Access

[373] Magnetic fields modulate blue-light-dependent regulation of neuronal firing by cryptochrome (2016)

Giachello, C. N. G., Scrutton, N. S., Jones, A. R., Baines, R. A.,

Journal of Neuroscience, 36 (42) 10742-10749; DOI: 10.1523/JNEUROSCI.2140-16.2016 Open Access

[372] Untangling heavy protein and cofactor isotope effects on enzyme- catalyzed hydride transfer (2016)

Longbotham, J. E., Hardman, S. J. O., Görlich, S., Scrutton, N.S., Hay, S.,

Journal of the American Chemical Society, 138 (41), 13693–13699, DOI: 10.1021/jacs.6b07852 Open Access

[371] Editorial overview: Catalysis and regulation: enzyme structure, mechanism, and biosynthetic pathways (2016) Christianson, D. W., Scrutton, N. S.

Current Opinion in Structural Biology, 41, viii-x, DOI: 10.1016/j.sbi.2016.09.005

[370] SYNBIOCHEM Synthetic Biology Research Centre, Manchester e A UK foundry for fine and speciality chemicals production (2016)

Le Feuvre R. A., Carbonell P., Currin A., Dunstan M., Fellows D., Jervis A. J., Rattray N. J. W., Robinson C. J., Swainston N., Vinaixa M., Williams A., Yan C., Barran P., Breitling R., Chen G. G., Faulon J. L., Goble C., Goodacre R., Kell D. B., Micklefield J., Scrutton N. S., Shapira P., Takano E., Turner N. J.

Synthetic and Systems Biotechnology, 1, 4, 271-275, DOI:10.1016/j.synbio.2016.07.001 Open Access

[369] A perspective on conformational control of electron transfer in nitric oxide synthases (2016)

Hedison, T. M., Hay, S., Scrutton, N.S.

Nitric Oxide, 63, 61-67, DOI: 10.1016/j.niox.2016.09.002 Open Access

[368] Donor–acceptor distance sampling enhances the performance of “Better than Nature” nicotinamide coenzyme biomimetics (2016)

Geddes, A., Paul, C. E., Hay,S., Hollmann, F., Scrutton, N. S.,

Journal American Chemical Society, 138, 11089-11092 , DOI: 10.1021/jacs.6b056252 Open Access

[367] Mass Spectrometry locates local and allosteric conformational changes that occur on co-factor binding (2016)

Beveridge, R., Migas, L., Payne, K., Scrutton, N.S., Leys, D., Barran, P.

Nature Communications, 7, 12163, DOI: 10.1038/ncomms12163 Open Access

[366] Pinpointing a mechanistic switch between ketoreduction and 'ene'-reduction in short chain dehydrogenases/reductases (2016)

Lygidakis, A., Karuppiah, V., Hoeven, R., Ní Cheallaigh, A., Leys, D., Gardiner, J. M., Toogood, H. S., Scrutton, N.S.,

Angewandte Chemie, 55, 9596-9600, DOI: 10.1002/anie.201603785, Open Access

[365] Correlating calmodulin landscapes with chemical catalysis in neuronal nitric oxide synthase using time-resolved FRET and a 5-deazaflavin thermodynamic trap. (2016)

Hedison, T. M., Leferink, N. G. H., Hay, S., Scrutton, N. S.

ACS Catalysis, 6, 5170-5180, DOI: 10.1021/acscatal.6b01280 Open Access

[364] A 'plug and play' platform for the production of diverse monoterpene hydrocarbon scaffolds in Escherichia coli (2016)

Leferink, N. G. H., Jervis, A. J., Zebec, Z., Toogood, H. S., Hay, S., Takano, E., Scrutton, N. S.,

Chemistry Select, 1, 1893-1896, DOI: 10.1002/slct.201600563 Open Access

[363] Towards synthesis of monoterpenes and derivatives using synthetic biology (2016)

Zebec, Z., Wilkes, J., Jervis, A. J., Scrutton, N. S., Takano, E., Breitling, R.

Current Opinion in Chemical Biology, 34, 37-43, DOI: 10.1016/j.cbpa.2016.06.002 Open Access

[362] SYNBIOCHEM – a SynBio foundry for the biosynthesis and sustainable production of fine and speciality chemicals (2016)

Carbonell, P., Currin, A., Dunstan, M., Fellows, D., Jervis, A., Rattray, N. J. W., Robinson, C. J., Swainston, N., Vinaixa, M., Williams, A., Yan, C., Barran, P., Breitling, R., Chen, G. G., Faulon, J-L., Goble, C., Goodacre, R., Kell, D. B., Le Feuvre, R., Micklefield, J., Scrutton, N. S., Shapira, P., Takano, E., & Turner, N. J.

Biochemical Society Transactions, 44, 675-677, DOI: 10.1042/BST20160009 Open Access

[361] Sweating the assets of flavin cofactors: new insight of chemical versatility from knowledge of structure and mechanism (2016)

Leys, D. & Scrutton, N. S.

Current Opinion in Structural Biology, 41, 19–26, DOI: 10.1016/j.sbi.2016.05.014 Open Access

[360] Multiple active site residues are important for photochemical efficiency in the light-activated enzyme protochlorophyllide oxidoreductase (POR) (2016)

Menon, B. R. K., Hardman, S. J. O. Scrutton, N. S., Heyes, D. J.

Journal of Photochemistry and Photobiology B: Biology, 161, 236-243, DOI: 10.1016/j.jphotobiol.2016.05.029 Open Access

[359] Ene reductases and their applications (2016)

Knaus, T., Toogood, H. S. & Scrutton, N. S.

In Biocatalysis – Green Technology (Ed., Patel, R). Wiley STM. 473-488. ISBN: 978-1-118-82229-6

[358] Natural product biosynthesis in Escherichia coli: Mentha monoterpenoids (2016)

Toogood, H. S, Tait, S., Jervis, A., Ní Cheallaigh, A., Humphreys, L., Takano, E., Gardiner, J. M., Scrutton, N.S.

Methods in Enzymology, 575, 247-270 , DOI:10.1016/bs.mie.2016.02.020

[357] Cross-species analysis of protein dynamics associated with hydride and proton transfer in the catalytic cycle of the light-driven enzyme protochlorophyllide oxidoreductase (2016)

Hoeven, R., Hardman, S. J. O., Heyes, D. J., Scrutton, N. S.

Biochemistry, 55(6), 903-913, DOI: 10.1021/acs.biochem.5b01355 Open Access

[356] Better than Nature: nicotinamide biomimetics that outperform natural coenzymes (2016)

Knaus, T., Paul, C. E., Levy, C. W., de Vries, S., Mutti, F. G., Hollmann, F., Scrutton, N. S.,

Journal of the American Chemical Society, 138, 1033-1039, DOI: 10.1021/jacs.5b12252 Open Access

[355] Light-driven biocatalytic reduction of α,β-unsaturated compounds by ene reductases employing transition metal complexes as photosensitizers (2016)

Peers, M.K., Toogood, H.S., Heyes, D.J., Mansel,l D., Coe, B.J., and Scrutton, N.S.

Catalysis Science & Technology, 6, 169-177, DOI: 10.1039/C5CY01642H Open Access

[354] Cyclometalated Ir(III) complexes of deprotonated N-methylbipyridinium ligands: effects of quaternised N centre position on luminescence (2015)

Coe, B., Helliwell, M., Raferty, J., Sanchez, S., Peers, M., Scrutton, N. S.

Dalton Transactions, 44, 20392-20405, DOI: 10.1039/C5DT03753K Open Access

[353] Conversion of alcohols to enantiopure amines through dual-enzyme hydrogen-borrowing cascades (2015)

Mutti, F. G., Knaus, T., Scrutton, N. S., Breuer, M., Turner, N. J.

Science, 349, 6255, 1525-2529. DOI: 10.1126/science.aac9283

[352] Structure and mechanism of a viral collagen prolyl hydroxylase (2015) Longbotham, J. E., Levy, C., Johannissen, L. O., Tarhonskaya, H., Jiang, S., Loenarz, C., Flashman, E., Hay, S., Schofield, C. J., Scrutton, N. S.

Biochemistry, 54 (39), 6093–6105, DOI: 10.1021/acs.biochem.5b00789 Open Access

[351] Real time analysis of conformational control in electron transfer reactions of human cytochrome P450 reductase with cytochrome c. (2015)

Hedison, T. M., Hay, S., Scrutton, N. S.

FEBS Journal, 282, 4357–4375, DOI: 10.1111/febs.13501 Open Access

[350] The photochemical mechanism of a B12-dependent photoreceptor protein (2015)

Kutta, R. J., Hardman, S. J. O., Johannissen, L. O., Bellina, B., Messiha, H. L., Ortiz-Guerrero, J. M., Elías-Arnanz, M., Padmanabhan, S., Barran, P., Scrutton, N. S., Jones, A. R.

Nature Communications, 6, 7907, DOI: 10.1038/ncomms8907 Open Access

[349] Water-soluble Ir(III) complexes of deprotonated N-methylbipyridinium ligands: Fluorine-free blue emitters (2015)

Coe, B., Helliwell, M., Sanchez, S., Peers, M., and Scrutton, N. S.

Dalton Transactions, 44, 15420-15423, DOI: 10.1039/C5DT02591E Open Access

[348] Special Issue: Flavins and Flavoproteins, Introduction (2015) Chaiyen, P. and Scrutton, N. S.

FEBS Journal, 282, 16, 3001-3002, DOI: 10.1111/febs.13337

[346] New cofactor supports α,β-unsaturated acid decarboxylation via 1,3-dipolar cycloaddition (2015)

Payne, K. A. P., White, M. D., Fisher, K., Khara, B., Bailey, S. S., Parker, D., Rattray, N. J. W., Trivedi, D. K., Goodacre, R., Beveridge, P., Barran, P., Rigby, S. E. J., Scrutton, N. S., Hay, S., & Leys, D.

Nature, 522, 497-501, DOI:10.1038/nature14560

[345] UbiX is a flavin prenyltransferase required for bacterial ubiquinone biosynthesis (2015)

White, M. D., Payne, K. A. P., Fisher, K., Marshall, S. A., Parker, D., Rattray, N. J. W., Trivedi, D. K., Goodacre, R., Rigby, S. E. J., Scrutton, N. S., Hay, S., & Leys, D.

Nature, 522, 502-506, DOI:10.1038/nature14559

[344] Enzymatic menthol production: one-pot approach using engineered Escherichia coli (2015)

Toogood, H., Cheallaigh, A. N., Tait, S., Mansell, D. J., Jervis, A., Lygidakis, A., Humphreys, L. D., Takano, E., Gardiner, J. M., & Scrutton, N. S.

ACS Synthetic Biology, 4(10), 1112–1123, DOI: 10.1021/acssynbio.5b00092 Open Access

[343] Catalytic mechanism of cofactor-free dioxygenases and how they circumvent spin-forbidden oxygenation of their substrates (2015)

Hernández-Ortega, A., Quesne, M. G., Bui, S., Heyes, D. J., Steiner, R. J., Scrutton, N. S., & de Visser, S. P.

Journal of the American Chemical Society. 137, 7474-7487, DOI: 10.1021/jacs.5b03836 Open Access

[342] Probing reversible chemistry in coenzyme B12‐dependent ethanolamine ammonia lyase with kinetic isotope effects (2015)

Jones, A. R., Rentergent, J., Scrutton, N. S. & Hay, S.

Chemistry A European Journal, 21, 8826–8831, DOI: 10.1002/chem.201500958 Open Access

[341] Syntheses and electronic and optical properties of complexes of the bis(2,2′-bipyrazyl)ruthenium unit (2015)

Coe, B. J., Peers, M. K., Scrutton, N. S.

Polyhedron, 96, 57-65, DOI: 10.1016/j.poly.2015.04.028 Open Access

[340] A microbial platform for renewable propane synthesis based on a fermentative butanol pathway (2015)

Menon, N., Pasztor, A., Menon, B. R. K., Kallio, P., Fisher, K., Akhtar, K., Leys, D., Jones, P. R. & Scrutton, N. S.

Biotech for Biofuels, 8, 61, DOI: 10.1186/s13068-015-0231-1 Open Access

[339] Nuclear quantum tunnelling in enzymatic reactions – an enzymologist’s perspective (2015)

Johannissen, L., Hay, S. & Scrutton, N. S.

Phys. Chem. Chem. Phys., 7, 30775-30782, DOI 10.1039/C5CP00614G Open Access

[338] Glutamate 338 is an electrostatic facilitator of C-Co bond breakage in a dynamic/ electrostatic model of catalysis by ornithine aminomutase (2015)

Menon, B., Menon, N., Fisher, K., Rigby, S. E. J., Leys, D. & Scrutton, N. S.

FEBS Journal 282, 1242-1255, DOI: 10.1111/febs.13215 Open Access

[337] Does the pressure dependence of kinetic isotope effects report usefully on dynamics in enzyme H-transfer reactions? (2015)

Hoeven, R., Heyes, D. J., Hay, S. & Scrutton, N. S.

FEBS Journal ,282, 16, 3243-3255 , DOI: 10.1111/febs.13193 Open Access

[336] Excited state charge separation in the photochemical mechanism of the light-driven enzyme protochlorophyllide oxidoreductase (2015)

Heyes, D. J., Hardman, S. J. O., Hedison, T. M., Hoeven, R., Greetham, G. M., Towrie, M. & Scrutton, N. S.

Angewandte Chemie, 54, 1512-1515, DOI: 10.1002/anie.201409881 Open Access

[335] Systematic methodology for the development of biocatalytic hydrogen-borrowing cascades: application to the synthesis of chiral α-substituted carboxylic acids from α-substituted α,β-unsaturated aldehydes (2015)

Knaus, T., Mutti, F. G., Humphreys, L. D., Turner, N. J. & Scrutton, N. S.,

Organic Biomolecular Chemistry, 13, 223-233, DOI: 10.1039/C4OB02282C Open Access

[334] Ordered multilayers of cytochrome P450 reductase adsorbed at Au(110) phosphate buffer interfaces (2015)

Smith, C. I., Convery, J. H., Khara, B., Scrutton, N. S. & Weightman, P.

Physica Status Solidi B: Basic Solid State Physics, 252, 181-186, DOI: 10.1002/pssb.201451221

[333] Towards the free energy landscape for catalysis in mammalian nitric oxide synthases (2015)

Leferink, N. G. F., Hay, S., Rigby, S. E. J. & Scrutton, N. S.

FEBS Journal,282, 16, 3016-3029, DOI: 10.1111/febs.13171